Student at Lycée Molière in Paris where she follows the theater option, Anne-Sophie Morillon then studied theater at Cours Florent, then Cours Périmony and Cours Eva Saint-Paul parallel to her university studies. Anne-Sophie Morillon starts under the gaze of Nicole Garcia who chooses her to play Laura Morante's daughter in a set of commercials for the pantyhose brand Le Bourget. Very quickly, she makes appearances in the feature films of Cédric Kahn, Patrice Leconte and she plays in many TV movies made by Gilles Béhat, Jérôme Boivin and Aline Issermann, among others.